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KMID : 0861020140290020001
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Korea Journal of Herbology
2014 Volume.29 No. 2 p.1 ~ p.6
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Anti-oxidant and anti-inflammatory activities of the various kinds of herbal tea
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Lee Jin-Wook
Eo Hyun-Ji
Park Gwang-Hun
Song Hun-Min
Woo So-Hee
Kim Mi-Kyoung
Eom Jung-Hye
Lee Man-Hyo
Lee Jeong-Rak
Koo Jin-Suk
Jeong Jin-Boo
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Abstract
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Objectives : Reactive oxygen species (ROS) are involved in a wide spectrum of diseases including chronic inflammation and cancer. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the extracts from the herbal teas such as Lonicera japonica Thunberg (L. japonica), Chrysanthemum morifolium Ramat (C. morifolium), Mentha arvensis L. (M. arvensis), and P.rhizoma.
Methods : Anti-oxidant activity was evaluated using DPPH radical scavenging assay and Fe2+ chelating assay. And DNA cleavage assay was performed to evaluate an anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell line.
Results : L. japonica scavenged DPPH radical by 9.8% at 12.5 μg/ml, 24.8% at 25 μg/ml, 34.3% at 50 μg/ml, 61.1% at 100 μg/ml and 75.8% at 200 μg/ml, respectively. In addition, C. morifolium and M. arvensis removed DPPH radical by 15.6% and 10.4% at 12.5 μg/ml, 34.8% and 22.8% at 25 μg/ml, 66.9% and 43.3% at 50 μg/ml, 87.4% and 69.1% at 100 μg/ml, and 92.1% and 73.2% at 200 μg/ml, respectively. However, P. rhizoma did not affect on DPPH radical scavenging. The Fe2+ chelating activity was highest in L. japonica, but lowest in P. rhizoma among the herbal teas. In addition, the extracts from L. japonica, C. morifolium and M. arvensis inhibited oxidative DNA damage via its anti-oxidant activity. In anti-inflammatory effect, the extracts from C. morifolium inhibited NO production. In addition, it suppressed the NF-κB signaling pathway in LPS-stimulated RAW 264.7 cells.
Conclusions : Together, this study indicates that L. japonica, M. arvensis and C. morifolium possess the protective effect against the oxidative DNA damage. Furthermore, C. morifolium exerts an anti-inflammatory effect.
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KEYWORD
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Herbal teas: Anti-oxidant, Oxidative damage, Anti-inflammation
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